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control human ngf  (Alomone Labs)


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    Alomone Labs control human ngf
    Control Human Ngf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+human+ngf/Human+P2Y11+Receptor+Blocking+Peptide/pmc02998128-309-16-12
    Average 90 stars, based on 4 article reviews
    control human ngf - by Bioz Stars, 2026-09
    90/100 stars

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    control human ngf  (Alomone Labs)
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    mouse monoclonal anti human b ngf antibody  (R&D Systems)
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    Figure 2 Immunocytological localization of <t>NGF</t> in human breast cancer cells. (a) Immunocytochemistry of NGF in normal (NBEC) and cancer breast epithelial cells (MCF-7 cells) performed on tissue culture dishes using <t>monoclonal</t> antibodies against NGF. Calibration bar represents 100 mm. (b) Fluorescence confocal micrographs showing breast cancer cells (MCF-7) and NBECs treated with or without ionomycin. Cellular localization of NGF was assessed using secondary antibodies directed against anti-NGF coupled to Alexa 488. Ionomycin caused the disappearance of vesicles containing NGF. The calibration bar represents 10 mm
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    Figure 2 Immunocytological localization of NGF in human breast cancer cells. (a) Immunocytochemistry of NGF in normal (NBEC) and cancer breast epithelial cells (MCF-7 cells) performed on tissue culture dishes using monoclonal antibodies against NGF. Calibration bar represents 100 mm. (b) Fluorescence confocal micrographs showing breast cancer cells (MCF-7) and NBECs treated with or without ionomycin. Cellular localization of NGF was assessed using secondary antibodies directed against anti-NGF coupled to Alexa 488. Ionomycin caused the disappearance of vesicles containing NGF. The calibration bar represents 10 mm

    Journal: Oncogene

    Article Title: Nerve growth factor overexpression and autocrine loop in breast cancer cells.

    doi: 10.1038/sj.onc.1206805

    Figure Lengend Snippet: Figure 2 Immunocytological localization of NGF in human breast cancer cells. (a) Immunocytochemistry of NGF in normal (NBEC) and cancer breast epithelial cells (MCF-7 cells) performed on tissue culture dishes using monoclonal antibodies against NGF. Calibration bar represents 100 mm. (b) Fluorescence confocal micrographs showing breast cancer cells (MCF-7) and NBECs treated with or without ionomycin. Cellular localization of NGF was assessed using secondary antibodies directed against anti-NGF coupled to Alexa 488. Ionomycin caused the disappearance of vesicles containing NGF. The calibration bar represents 10 mm

    Article Snippet: They were then incubated overnight at 41C with a mouse monoclonal anti-human b-NGF antibody (R&D System), followed by 2 h at room temperature with biotinylated antimouse IgG antibodies diluted 1/250 and 2 h at room temperature with avidin/biotin–peroxidase complex diluted 1/200.

    Techniques: Immunocytochemistry, Bioprocessing, Fluorescence

    Figure 5 NGF autocrine stimulation of breast cancer cell growth. (a) Time-dependent inhibition of basal breast cancer cell growth in a serum-free medium (&) with 1 mg/ml MAB-256 neutralizing anti- NGF (n) or with 10 nM K-252a ( ). Cells were counted after trypsinization with a hemocytometer. No modification of cellular growth was observed with control antibodies (not shown). (b) Dose effect of K-252a on MCF-7 cell growth and (c) dose effect of neutralizing anti-NGF. Cells were plated at 10 000/ml, and after 24 h of incubation in a serum-free medium, the indicated concentrations of anti-NGF and K-252a were added. Results presented in (a–c) represent the means7s.d. of five independent experiments

    Journal: Oncogene

    Article Title: Nerve growth factor overexpression and autocrine loop in breast cancer cells.

    doi: 10.1038/sj.onc.1206805

    Figure Lengend Snippet: Figure 5 NGF autocrine stimulation of breast cancer cell growth. (a) Time-dependent inhibition of basal breast cancer cell growth in a serum-free medium (&) with 1 mg/ml MAB-256 neutralizing anti- NGF (n) or with 10 nM K-252a ( ). Cells were counted after trypsinization with a hemocytometer. No modification of cellular growth was observed with control antibodies (not shown). (b) Dose effect of K-252a on MCF-7 cell growth and (c) dose effect of neutralizing anti-NGF. Cells were plated at 10 000/ml, and after 24 h of incubation in a serum-free medium, the indicated concentrations of anti-NGF and K-252a were added. Results presented in (a–c) represent the means7s.d. of five independent experiments

    Article Snippet: They were then incubated overnight at 41C with a mouse monoclonal anti-human b-NGF antibody (R&D System), followed by 2 h at room temperature with biotinylated antimouse IgG antibodies diluted 1/250 and 2 h at room temperature with avidin/biotin–peroxidase complex diluted 1/200.

    Techniques: Inhibition, Control, Incubation